Doctoral Dissertation in Medical Sciences Proves the Potential of Purple Sweet Potato Ethanol Extract in the Treatment of Vitiligo Sufferers

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• Senin, 20 Mei 2024
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Doctoral Dissertation in Medical Sciences Proves the Potential of Purple Sweet Potato Ethanol Extract in the Treatment of Vitiligo Sufferers

Located in Dr. A A. Made Djelantik, Faculty of Medicine Building, Udayana University, Denpasar, the Doctoral Promotion exam took place with Promovenda candidate Dr. Ni Nyoman Ayu Sutrini, M.Repro., Sp.KK., FINSDV., FAADV., with the dissertation title "The Effect of Giving Ethanol Extract of Purple Sweet Potato (Ipomoea Batatas L) on Global DNA Methylation Levels, Catalase, Tyrosinase, and CXCL10 Levels in the Serum of Vitiligo Patients Treated with Excimer Light 308 nm." (16/5/2024)

Vitiligo is a multifactorial skin disease caused by loss of melanin. The pathomechanism is still being investigated because many of the problems caused have not been resolved. The role of epigenetic mechanisms in vitiligo, such as DNA methylation, is receiving increasing attention in addition to oxidative stress, autoimmune and genetic factors. This study aims to explore the interaction between oxidative stress, epigenetics, autoimmune and genetic susceptibility through epigenetic mechanisms of purple sweet potato by simultaneously measuring global DNA methylation levels, levels of the antioxidant enzyme Catalase (CAT), the melanogenesis enzyme Tyrosinase (TYR) and the chemokine CXCL10 in serum vitiligo sufferers treated with Excimer light (EL) 308 nm.

This experimental research with a randomized pre and post-test control group design involved 24 research subjects who were divided into two groups. The treatment group was given 400 mg purple sweet potato ethanol extract capsules, while the control group was given capsules that did not contain purple sweet potato ethanol extract. All subjects in both groups took capsules twice daily for 8 weeks and received EL 308 nm therapy. Blood serum samples were taken before and after treatment for ELISA examination of global DNA methylation levels, CAT, TYR and CXCL10, and also assessed vitiligo activity using the Vitiligo Disease Activity score (VIDA) and vitiligo severity using the Vitiligo Area Scoring Index (VASI). . Data analysis used Independent T-test, Mann-Whitney, chi-square, Fisher's exact, linear regression and path analysis.

The results of the analysis showed that administration of purple sweet potato ethanol extract significantly increased CAT levels and TYR levels, significantly reduced VIDA scores and VASI scores, but did not significantly increase global DNA methylation levels and CXCL10 levels. Pathway analysis showed that purple sweet potato extract directly influenced CAT (B: 0.273; p: 0.020), TYR (B: 0.548; p: 0.006) and VASI (B: 0.516; p: 0.010) enzyme levels, but not through the influence of levels. global DNA methylation. Meanwhile, global DNA methylation levels directly influenced CXCL10 levels (B: -0.402; p: 0.049). Regarding clinical outcomes, only TYR levels affected VIDA (B: -0.488; p: 0.016).

 

The results of this study conclude that the use of epigenetic mechanisms of purple sweet potato in this study does not prove the existence of interactions between oxidative stress, epigenetic, autoimmune and genetic susceptibility factors in the pathomechanism of vitiligo. However, it proves that there is a direct relationship and interaction between purple sweet potato ethanol extract as an antioxidant and increasing the antioxidant enzyme Catalase, increasing the melanogenesis enzyme Tyrosinase and the severity of vitiligo without changing global DNA methylation levels. The decrease in vitiligo activity was obtained from a direct relationship with an increase in TYR levels. There are interesting findings where there is a direct relationship and interaction between global DNA methylation levels only with CXCL10 levels.

This exam was led by the Deputy Dean for Academic Affairs and PLANNING FK Unud Dr. Dr. I Gede Eka Wiratnaya, Sp.OT (K), with a team of examiners:

1. Prof. Dr. Dr. A A. Wiradewi Lestari, Sp.PK, Subsp.BDKT(K), Subsp.KV(K) (Promoter)

2.Dr. Dr. I.G.A.A. Praharsini, Sp.KK., FINSDV (Copromoter I)

3.Dr. Dr. Sianny Herawati, Sp.PK, Subsp.BDKT(K), Subsp.NR(K) (Copromotor (II)

4. Prof. Dr. Dr. I Made Bakta, Sp.PD-KHOM

5. Prof. Dr. Dr. I Made Jawi, M. Kes

6. Prof. drh. I Nyoman Mantik Astawa, Ph.D

7. Prof. Dr. Ir. I.B. Manuaba's son, M. Phill

8.Dr. Dr. Luh Made Mas Rusyati, Sp.KK (K)

9.Dr. Dr. Agung Wiwiek Indrayani, S.Ked., M.Kes

10.Dr. Dr. HE. Ika Wahyuniari, M. Kes

Meanwhile, academic invitations are:

1.Dr. Dr. Ketut Kwartantaya Winaya, Sp.KK., FINSDV, FAADV

2.Dr. Dr. Kadek Mulyantari, Sp.PK, Subsp.BDKT(K)

3. Dr. Dr. Nyoman Suryawati, M.Kes., Sp.KK(K)., FINSDV, FAADV

4.Dr. Dr. I Gst. Ayu Agung Dwi Karmila, Sp. KK(K), FINSDV, FAADV

5.Dr. Dr. I Gst. Ayu Agung Elis Indira, Sp. KK(K), FINSDV, FAADV

In this exam, Dr. Dr. Ni Nyoman Ayu Sutrini, M.Repro., Sp.KK., FINSDV., FAADV., was declared to have graduated as the 415th Doctoral Graduate of the Medical Science Doctoral Degree Study Program, Faculty of Medicine, Udayana University with the title Cumlaude