Doctoral Dissertation in Medical Sciences Reveals the Potential of Purple Sweet Potato Extract in Overcoming Obesity

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• Minggu, 23 Juni 2024
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Doctoral Dissertation in Medical Sciences Reveals the Potential of Purple Sweet Potato Extract in Overcoming Obesity

Located in Dr. A A. Made Djelantik Lt. IV Denpasar Faculty of Medicine Building, the Doctoral Promotion exam took place with the promovendus candidate, dr. I Wayan Surudarma, M.Sc., with the dissertation title "The Effect and Mechanism of Action of Purple Sweet Potato Tuber Extract (Ipomoea batatas L.) on the Levels of MDA, TNF-α, Leptin and p-AMPK in Inhibiting Weight Gain in Male Wistar Rats High Fat Diet Induced."(6/21/2024)

Obesity is a major health problem throughout the world. Several studies confirm that consumption of foods rich in anthocyanins can prevent obesity in healthy subjects and can reduce body weight in obese subjects. This study aims to prove the effect and mechanism of purple sweet potato tuber extract on levels of malondialdehyde (MDA), TNF-α, leptin, phosphorylated AMPK (p-AMPK) in inhibiting the increase in body weight of male Wistar rats induced by a high-fat diet.

The design of this research is the randomized post test only control group design. A total of 30 male Wistar rats that met the eligibility criteria were randomized into 3 groups, namely treatment, control and normal. The treatment group was given a high-fat diet and 1 ml of 100 mg/ml purple sweet potato tuber extract for 12 weeks. The control group was given a high-fat diet and 1 ml of saline, while the normal group was given a standard diet and 1 ml of saline. MDA, TNF-α, leptin and p-AMPK levels were examined using enzyme-linked immunosorbent assay (ELISA). The data obtained were then subjected to descriptive and inferential statistical analysis, as well as path-analysis.

The results showed that the mean MDA level in the treatment group was lower than the control group (p=0.002). The mean TNF-α level in the treatment group was lower than the control (p= 0.038). The mean leptin level of the treatment group was lower than the control (p=0.002). The mean p-AMPK level in the treatment group was higher than the control (p=0.001). The mean body weight of the treatment group was lower than the control (p=0.024). Giving purple sweet potato tuber extract had a direct effect on reducing MDA levels (B: 0.79), TNF-α (B: 0.43) and leptin (B: 0.38), but had no direct effect on p-AMPK levels. as well as body weight. An inverse relationship was found between p-AMPK and levels of leptin (B: -0.44), TNF-α (B: -0.21) and MDA (B: -0.07). There was also a direct relationship between p-AMPK levels and weight loss (B: 0.06).

Thus, it can be concluded that purple sweet potato tuber extract has been proven to inhibit the increase in body weight of male Wistar rats induced by a high-fat diet through the mechanism of increasing p-AMPK levels due to decreasing levels of leptin, TNF-α and MDA.

This exam was led by the Dean of the Faculty of Medicine, Udayana University, Prof. Dr. Dr. Komang Januartha Putra Pinatih, M.Kes., with a team of examiners:

1. Prof. Dr. Dr. I Wayan Weta, MS., Sp.GK (Promoter)

2.Dr. Dr. Urged Made Wihandani, M.Kes (Copromotor I)

3. Dr.rer.nat. Dr. Ni Nyoman Ayu Dewi, M.Si (Copromotor II)

4. Prof. Dr. Dr. I Wayan Putu Sutirta Yasa, M.Si

5. Prof. Dr. Dr. I Gusti Ayu Sri Mahendra Dewi, Sp.P.A., Subsp.O.G.P.(K)

6.Dr. Dr. Ni Putu Sriwidyani, Sp.PA

7.Dr. Dr. Putu Anda Tusta Adiputra, Sp.B.Subsp.Onk(K)

8.Dr. Dr. Sianny Herawati, Sp.PK, Subsp.BDKT(K), Subsp.NR(K)

9.Dr. Dr. I Putu Eka Widyadharma, M.Sc., Sp.S(K)

Meanwhile, academic invitations are:

1.Dr. Dr. Luh Putu Ratna Sundari, M. Biomed

2.Dr. Dr. Syuma Adhy Awan, M.Kes., Sp.GK(K)

3.Dr. Dr. Agung Wiwiek Indrayani, S.Ked., M.Kes

4.Dr. Dr. I Wayan Sugiritama, M.Kes

5. dr. Made Winarsa Ruma, S.Ked., Ph.D

In this exam, Dr. Dr. I Wayan Surudarma, M.Si., was declared to have graduated as the 418th Doctoral Graduate of the Medical Science Doctoral Degree Study Program, Faculty of Medicine, Udayana University with the title of Very Satisfactory.