Doctor of Medical Sciences Studies the Potential of Adipose-Derived Mesenchymal Stem Cells in the Management of Pelvic Floor Dysfunction in Postmenopausal Women.

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• Jumat, 14 Maret 2025
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Doctor of Medical Sciences Studies the Potential of Adipose-Derived Mesenchymal Stem Cells in the Management of Pelvic Floor Dysfunction in Postmenopausal Women.

Held in the Postgraduate Hall, 3rd Floor of the Postgraduate Building, Udayana University, the Doctoral Program in Medical Sciences, Faculty of Medicine, Udayana University held an open Doctoral Promotion examination with the candidate for promovendus, dr. Kadek Fajar Marta, M.Biomed., Sp.OG, Subsp.Urogin-Re., with the dissertation title "The Effect of Adipose-Derived Mesenchymal Stem Cell (ADSC) Administration on the Expression of Collagen I, Collagen III, and Elastin in the Anterior Vaginal Wall of Post-Ovariectomy Menopausal Rat Models." (12/3/2025)

Pelvic floor dysfunction (PDL) in menopausal women is generally treated after symptoms appear, with conservative therapy or surgery. Adipose-Derived Mesenchymal Stem Cell (ADSC) is a potential prevention alternative because of its regenerative ability that plays a role in the formation of collagen and elastin for tissue repair processes. This study aims to determine the effect of ADSC administration on the expression of collagen I, collagen III, and elastin in the anterior vaginal wall of post-ovariectomy menopause model rats. This study involved 18 female Wistar white rats with post-ovariectomy menopause models which were randomly divided into three groups, each consisting of 6 rats: a control group without ADSC administration, an intervention group with ADSC administration at a dose of 1×10⁶ cells, and a group with ADSC administration at a dose of 2×10⁶ cells. Samples of the anterior vaginal wall from each group were taken and immunohistochemical examination was performed. The research outputs included the expression of collagen I, collagen III, elastin, and the ratio of collagen I and collagen III in pixel intensity units. Data analysis using IBM SPSS version 22.

The results of the analysis showed a significant difference between the control and intervention groups, marked by higher collagen I and elastin expression and lower collagen III expression in the intervention group with p values ​​of 0.002 each. In addition, the results of the analysis of the ratio of collagen I and collagen III also showed a significant difference between the control and intervention groups with p values ​​of 0.005.

Administration of ADSCs to post-ovariectomy menopause model mice has an effect on increasing collagen I expression, decreasing collagen III expression, and increasing elastin expression in the anterior vaginal wall.

These findings indicate the potential of ADSCs as a therapy that can contribute to the management of pelvic floor dysfunction in postmenopausal women.

This examination was led by the Dean of the Faculty of Medicine, Udayana University, Prof. Dr. dr. Komang Januartha Putra Pinatih, M.Kes., with the following examiner team:

1. Prof. Dr. dr. I Wayan Putu Sutirta Yasa, M.Si (Promoter)

2. Dr. dr. A.A. Ngurah Jaya Kusuma, Sp.OG, Subsp.K.Fm, MARS (Copromotor I)

3. Prof. drh. I Nyoman Mantik Astawa, Ph.D (Copromoter II)

4. Prof. Dr. Dr. I Made Jawi, M. Kes

5. Prof. Dr. Dr. I Made Sudarmaja, M. Kes

6.Dr. Dr. Ni Made Linawati, M.Sc

7.Dr. Dr. I Gede Mega Putra, Sp.OG., Subsp.Urogin Re

8.Dr. Dr. I Nyoman Gede Budiana, Sp.OG., Subsp-Onk

9.Dr. drh. Ida Bagus Kade Suardana, M.Si

10.Dr. Dr. I Nyoman Bayu Mahendra, Sp.OG., Subsp-Onk., M.Hkes

By academic invitation:

1.Dr. Dr. I Gusti Putu Hendra Sanjaya, Sp.B., Sp.BP-RE., Subsp.L.BL(K)

2.Dr. Dr. I Made Pande Dwipayana, Sp.PD-KEMD

3.Dr. Dr. I Gede Ngurah Harry Wijaya Surya, Sp.OG., Subsp.Obsos

4.Dr. Dr. I Putu Eka Widyadharma, M.Sc, Sp.N(K)

5.Dr. Dr. Kadek Budi Santosa, Sp.U(K)

In this exam, Dr. Dr. Kadek Fajar Marta, M.Biomed., Sp.OG, Subsp.Urogin-Re., was declared to have graduated as the 449th Doctoral Graduate of the Medical Science Doctoral Study Program, Faculty of Medicine, Udayana University with Cumlaude predicate.

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