Doctoral Research in Medical Sciences Examines the Potential Benefits of Purple Sweet Potato Extract on Lung Fibrosis Due to Silica Dust

• Posted by: Admin
• Jumat, 12 Juli 2024
• Has been read 563 time(s)

Doctoral Research in Medical Sciences Examines the Potential Benefits of Purple Sweet Potato Extract on Lung Fibrosis Due to Silica Dust

Located in the Postgraduate Hall, Fl. III Udayana University Postgraduate Building, the Doctoral Promotion exam took place with the promovendus candidate, dr. I Gusti Ngurah Bagus Artana, Sp.PD-KP, FINASIM., with the dissertation title "The Effect of Giving Purple Sweet Potato Ethanol Extract on Levels of Tumor Necrosis Factor-α, Superoxide Dismutase, and Transforming Growth Factor-β, as well as Lung Inflammation and Fibrosis Scores in Wistar Rats Exposed to Silica Dust." (11/7/2024)

The pathogenesis of pulmonary fibrosis due to silica is closely related to inflammation and oxidative stress. One alternative therapy for pulmonary fibrosis due to silica can be considered by administering herbal ingredients which have antioxidant and anti-inflammatory properties. A plant that has antioxidant and anti-inflammatory properties is purple sweet potato (Ipomoea batatas L.). This study aims to prove the role of oxidative stress and inflammation in the pathogenesis of respiratory system disorders after exposure to silica dust by administering antioxidants which have activities to suppress oxidative stress, inflammation and anti-fibrotic activity (by looking at levels of SOD, TNF-α, TGF-β, features of inflammation and fibrosis ) due to exposure to silica dust.

This study used Wistar rats with a randomized post-test only control group design. This study used 18 Wistar rats each in both groups. Rats induced by intratracheal silica were given 200 mg/0.5 mL of purple sweet potato ethanol extract for 14 days. On the 15th day, tissue samples were taken. SOD examination using spectrophotometry, TNF-α and TGF-β using ELISA technique, inflammation and fibrosis scores using histo-pathological-anatomical examination. Statistical analysis for TNF-α using the independent sample t-test, SOD and TGF-β using the Mann Whitney U test, and lung histopathological scores using the Chi square test. Multivariate analysis with path analysis.

Lung SOD levels in the treatment group were higher (2.355±0.195 ng/mL vs. 0.448±0.143 ng/mL; p< 0.001). Lung TNF-α levels in the treatment group were lower (58.92±9.05 ng/L vs. 133.71±8.04 ng/L; p< 0.001). Lung TGF-β levels in the treatment group were lower (41.39±9.17 ng/L vs. 97.19±11.5 ng/L; p< 0.001). The mean lung inflammation score in the treatment group was lower (2.46 ± 0.39 vs. 3.51 ± 0.49; p < 0.001). The mean lung fibrosis score in the treatment group was lower (3.22±0.31 vs. 4.93±0.39; p< 0.001).

Ethanol extract of purple sweet potato reduces oxidative stress and suppresses inflammation in the lungs due to exposure to silica dust, as evidenced by the following findings. Lung SOD levels were higher when given purple sweet potato extract, levels of TNF-α, TGF-β, inflammation score, and lung fibrosis were lower when given purple sweet potato extract. Future studies are recommended in more thoroughly studying other signaling pathways in the protective mechanisms of purple sweet potato.

This exam was led by the Deputy Dean for Student Affairs and Information, Faculty of Medicine, Udayana University, Prof. Dr. I Made Ady Wirawan, S.Ked, MPH., Ph.D., with a team of examiners:

1. Prof. Dr. Dr. I Made Jawi, M.Kes (Promoter)

2. Prof. Dr. Dr. Ketut Suryana, Sp.PD-KAI., FINASIM (Copromotor I)

3.Dr. Dr. Agung Wiwiek Indrayani, M.Kes (Copromotor II)

4. Prof. Dr. I Made Ady Wirawan, S.Ked., MPH., Ph.D

5.Dr. Dr. Urged Made Wihandani, M.Kes

6. dr. I Wayan Sumardika, M.Med.Ed., PhD

7.Dr. Dr. I Gusti Lanang Sidiartha, Sp.A(K)

8.Dr. Dr. I Wayan Suranadi, Sp.An-TI, Subsp. T.I. (K)

9.Dr. Dr. I Putu Eka Widyadharma, M.Sc., Sp.S(K)

10.Dr. Dr. I Made Muliarta, S.Ked., M.Kes

Meanwhile, academic invitations are:

1.Dr. Dr. Ida Ayu Ika Wahyuniari, M.Kes

2.Dr. Dr. I Gusti Ayu Artini, MSc

3.Dr. Dr. I Gusti Agung Gede Utara Hartawan, Sp.An., MARS., SH

4.Dr. Dr. Made Agus Hendrayana, M.Ked

5.Dr. Dr. I Made Pande Dwipayana, Sp.PD-KEMD FINASIM

In this exam, Dr. Dr. I Gusti Ngurah Bagus Artana, Sp.PD-KP, FINASIM., was declared to have graduated as the 425th Doctoral Graduate of the Medical Science Doctoral  Degree Study Program, Faculty of Medicine, Udayana University with the title VERY SATISFACTORY.

Gallery