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Doctor of Medicine Findings: Vitamin D Supplementation Effectively Increases Anti-Inflammatory Gene Expression in Obese Women
Doctor of Medicine Findings: Vitamin D Supplementation Effectively Increases Anti-Inflammatory Gene Expression in Obese Women
Located in Dr. A.A Made Djelantik Building of the Faculty of Medicine, Udayana University, Sudirman Campus, Denpasar, Bali, the Doctoral Promotion exam has taken place with Promovenda candidate Dr. Linda Sinto, MARS., M.Biomed (AAM) with the dissertation title "Giving Vitamin D Supplementation Increases FoxP3 mRNA Expression and IL-10 Levels, But Does Not Reduce IFN-ɣ Levels in Obese Adult Women." (26/3/2024)
Obesity is one of the major health challenges of this century. Central obesity causes mild chronic inflammation in the body and disturbances in immune system regulation. Vitamin D as an immunomodulator has a role in overcoming inflammation. The aim of this study is to prove that giving Vitamin D to obese female subjects can increase FoxP3 mRNA expression, IL-10 levels and reduce IFN-γ levels, as inflammatory parameters.
This research was an experimental study (randomized pre-test and post-test control group design) on 44 centrally obese women (BMI >25kg/m2 and abdominal circumference ≥80cm). Sampling was carried out consecutively based on the arrival of research subjects who met the research criteria at the Gracia-East Jakarta clinic. The research subjects were divided randomly into two groups, namely the control group (22 people) and the treatment group (22 people). The treatment group was given a dose of 5000 IU/day of vitamin D, while the control group was given a placebo. The intervention in both groups was carried out for 3 months. The 25(OH) Vitamin D levels, FoxP3 mRNA expression, IL-10 levels and IFN-γ levels, daily food intake and anthropometric data were examined, before and after the intervention.
After the intervention, FoxP3 mRNA expression increased significantly in the treatment group, whereas there was no significant change in the control group. Changes in mRNA expression in the treatment group were significantly higher than those in the control. IL-10 levels increased significantly in the treatment group (116 (35.4) to 205 (121); p = 0.005), whereas in the controls there was no significant change (p = 0.876). Changes in IL-10 levels in the treatment group were significantly higher than those in the control group. The decrease in IFN-γ levels was significant in the treatment group (27.4 (14.9) to 17 (7.06); p= 0.001), as well as in the control group (23.6 (13.6) to 14.3 ( 17.2); p=0.016). Changes in IFN-γ levels were not significantly different between the treatment groups compared to the control group.
The conclusion of this study is that giving 5000 IU Vitamin D supplementation to obese women can significantly increase FoxP3 mRNA expression and IL-10 levels through the anti-inflammatory mechanism of Vitamin D, but does not significantly reduce IFN-γ levels.
This exam was led by the Dean of FK Unud Prof. Dr. Dr. Komang Januartha Putra Pinatih, M.Kes., with a team of examiners:
1. Prof. Dr. Dr. I Wayan Weta, MS., Sp.GK (Promoter)
2. Prof. Dr. Dr. Bagus Komang Satriyasa, M. Repro (Copromotor I)
3. Prof. drh. I Nyoman Mantik Astawa, Ph.D (Copromoter II)
4. Prof. Dr. Dr. I Made Bakta, Sp.PD-KHOM
5. Prof. Dr. Dr. Wimpie I Pangkahila, Sp.And-KSAAM
6.Dr. Dr. I Gusti Ayu Agung Praharsini, Sp.KK., FINSDV
7.Dr. Dr. Made Ratna Saraswati, Sp.PD, KEMD., FINASIM
8. Dr.dr.Desak Made Wihandani, M.Kes
9.Dr. Dr. Ida Ayu Ika Wahyuniari, M.Kes
10. Dr. dr. I Made Muliarta, S.Ked., M.Kes
Meanwhile, academic invitations are:
1. Dr. Dr. Ni Made Linawati, M.Sc
2. Dr. Dr. Ni Putu Sriwidyani, Sp. PA
3.Dr. Ni Ketut Sutiari, SKM., M.Sc
4. dr. Ni Wayan Arya Utami, M.App.BSc.,Ph.D
5.Dr. Kadek Tresna Adhi, SKM., M.Kes
In this exam, Dr. Dr. Linda Sinto, MARS., M.Biomed (AAM), was declared to have graduated as the 410th Doctoral Graduate of the Medical Science Doctoral Degree Study Program with cum laude predicate.
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